Physical mapping of a male-sterile locus in Brassica

Physical mapping of a male-sterile locus in Brassica

Physical mapping of a male-sterile locus in Brassica

Spontaneous male sterility is an advantageous trait for both producing hybrids and understanding the developmental process of the male reproductive unit in many crops. Lu et al. use a map-based cloning strategy together with comparative mapping to study the triallelic genetic male-sterile locus BnMs5 in Brassica napus, and delimit it to a 21-kb fragment of the A8 chromosome. Sequence analysis suggests that BnMs5 originates from the B. rapa MF2 subgenome, and may reside in a hotspot region of chromosomal evolution. This work paves the way for further cloning of BnMs5, and presents a powerful method for mapping loci in plants with complex genomic architecture.

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